Fig. 4.

Serial changes of annexin V⁺ cells in normal PMNs cultured with acute KD serum. After isolated PMNs from healthy children and KD patients were cultured in vitro with acute KD serum or FBS for 24 and 48 h, the spontaneous apoptotic PMNs (annexin V⁺PI⁺ cells) were analysed using a flow cytometer. The data were expressed as the mean of samples ± s.e. from five experiments. •, Acute KD PMNs + FBS; ○, HC PMNs + acute KD serum; □, HC PMNs + FBS.