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IFN-γ secretion of unseparated peripheral blood mononuclear cells. PBMCs from (□) healthy volunteers (n = 7) and (▪) patients with allergic asthma (n = 6) were stimulated for 24 h with anti-CD3 antibodies (20 µg/ml) or PMA (10−8 m) and calcium-ionophore (10−6 m). Release of IFN-γ in culture supernatant fluids was detected by ELISA (pg/ml). Values are mean ± s.e.m.
