Fig. 1.

Expression of wild-type and mutant CD11/CD18 antigens on COS-7 transfectants. Wild-type CD18 cDNA or CD18 cDNA with the A270V, A341P, C590R and R593C mutations were transfected into COS-7 cells together with the wild-type cDNA of CD11a, CD11b or CD11c. CD11/CD18 expression was monitored by flow cytometry with the MoAb MHM23. The background histograms (shadow) are those obtained with transfectants of CD11a, CD11b or CD11c alone. Expression profiles of the CD11/CD18 transfectants are shown in solid lines. The subtracted profiles, using the CellQuest software, are shown in bold lines. Percentage of gated positive cells (%GP) in the subtracted profiles, the mean fluorescence intensity (MFI) and the expression index (EI) = %GP × MFI are also shown.