Table 1.

The effects of rolipram (10⁻⁸–10⁻⁵ m) with or without forskolin (10⁻⁴ m), and dexamethasone (10⁻⁶ m) on PHA-induced cAMP levels of PBMC

Treatments		cAMP (fmol/106 cells)
Spontaneous
Vehicle		10·6 ± 3·4
PHA stimulation
Vehicle		11·1 ± 3·3
Rolipram	10−8m	12·8 ± 3·4e
	10−7m	13·8 ± 4·0e
	10−6m	19·3 ± 4·6e
	10−5m	28·0 ± 6·0b
Dexamethazone	10−6m	6·8 ± 2·4e
PHA stimulation with forskolin (10−4m)
Rolipram	10−8m	18·4 ± 10·0e
	10−7m	25·6 ± 9·8a
	10−6m	36·8 ± 10·4b,c
	10−5m	56·0 ± 12·1b,d

 PBMC were incubated with the various drugs for 10 min and then stimulated with PHA (10 µg/ml) for 0·5 h. Each result is the mean ±SEM of 5 experiments.

^aP < 0·05,

^bP < 0·01 vs. PHA-stimulated vehicle;

^cP < 0·05,

^dP < 0·01 vs. corresponding rolipram with PHA;

^eno significant difference from PHA-stimulated vehicle (Student's t-test).