Table 2.
Outcome of Cryptosporidium parvum infection in TNFR/Tnfsf5 knockouts *
| Disrupted gene | No. studied | Mean (range) duration of infection | Peak ELISA O.D. | Gross appearance of bile ducts |
|---|---|---|---|---|
| None (control) | 6 | 3 (2–4)weeks | 0·02 | Normal |
| CD40−/− | 8 | >12 weeks | 1·6 | Sclerosed |
| Tnfsf5−/− | 8 | >12 weeks | 2·0 | Sclerosed |
| Tnfrsf1a−/− | 5 | 4 (3–5)weeks | 0·22 | Normal |
| Tnfrsf1b | 4 | 3·5 (3–4) weeks | 0·32 | Normal |
| Tnfrsf1a & 1b | 4 | 4 (3–5)weeks | 0·36 | Normal |
| Tnfrsf1a & Tnfsf5 | 4 | >12 weeks | 2·0 | Sclerosed |
| Tnfrsf1b & Tnfsf5 | 5 | >12 weeks | 2·0 | Sclerosed |
| Tnfrsf1a & 1b & Tnfsf5 | 5 | >12 weeks | 2·0 | Normal |
| Tnfrsf1a & 1b & CD40 | 4 | >12 weeks | 1·0 | Normal |
*
Mice were infected at 4 weeks of age and followed by weekly ELISA tests on stool. CP infection was defined by an O.D. > 0·1 and the duration of infection indicates the mean number of weeks for the O.D. to return to <0·05. All mice were euthanized 12 weeks after infection and the macroscopic appearance of their extrahepatic bile ducts recorded.