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RT-PCR analysis of expression of MIP-3α in human gingiva. Total RNA was prepared from gingival biopsies from three normal donors and seven periodontal disease patients. RT-PCR analysis was carried out for MIP-3α and GAPDH as described in Methods. Lanes 1 and 9: marker; lanes 2–8: gingival biopsies from periodontal diseased patients; lanes 10–12: gingival biopsies from normal donors.
