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Specificity of CpG ODN sequence in stimulating NO production. RAW 264·7 macrophages (1 × 106 cells/well) were incubated with of CpG ODN 1826, 1982, 1585, 2006 or E. coli DNA at a final concentration of 1 μg/ml for 24 h. Nitrite in the supernatant was determined after 24h of incubation. Unstimulated cells served as control. Data represent means and s.d. of three separate experiments each carried out in triplicate.
