Fig. 5.

Time required for maximal stimulation of NO in CpG ODN 1826 or LPS activated macrophages. RAW 264·7 macrophages (1 × 10⁶ cells/well) were activated with CpG ODN 1826 (1 μg/ml) (•) or LPS (10 ng/ml) (○) for different time intervals. At times indicated, the supernatant was discarded, washed three times with PBS before incubating further in fresh media. At 24 h, the supernatant was analysed for nitrite (a). Data represent mean and s.d. for three separated experiments, each carried out in duplicate. The cells were analysed for iNOS by immunoblotting (b).