Fig. 2.

Defect in the transport of peptides. (a) EBV-B cells HA-L and ST-EMO were infected with recombinant vaccinia viruses expressing the TAP1 (thin line) or TAP2 subunit (bold line). Cells were stained with the MoAb W6/32, or an isotype control (grey histogram). (b) HA-L cells were incubated at 27°C in the presence (27/1, 27/2) or absence (27/0) of exogenous peptide 1(IPSHSSNTAL) or peptide 2 (KVKQAFNPL). Controls included cells incubated at 37°C (37) and a hemizygous cell line (HAT). Cells were stained with the anti-HLA-Bw6 MoAb 126.39 or the anti-HLA-B7 MoAb BB7.1. Ratios of the mean fluorescence intensity (expressed in percent) after staining with BB7.1 compared with 126.39 were determined under the different conditions.