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Modulation of intracellular IL-1ra (a), IL-1β (b) and IL-1ra : IL-1β ratio (c) by cytokines in oral mucosal cells. Cells were incubated for 24 h with RPMI 1640 alone as control (C), IL-1α, TNFα, IFNγ, IL-10, TGFβ1, IL-4, IL-6, hydrocortisone (HC) or calcium. The concentration of cytokines in culture lysates was determined by ELISA. The results represent the mean of two experiments, each performed from different cultures. P < 0·05.
