Table 1.
Average mass (M + H+)* | 1514·6‡ | 869·4 | 1406·9 | 1439·6 |
Peptide sequence † | HIDTAEF | SPAQVVIR | WDLQHGLVTIPK | IQENFNVWDFK |
| | | | | :| | |: | | | | | | | | | | | | | | | | | | | | |: | |
Hypothetical oxido reductase (Q46857) | ..RSIDTAAAY… | KTPAQIVIRW… | RWHLDSGLVVIPKSVTPSRIAENFDVWDFRL.. | |
Average mass (M + H+)* | 1506·6 | |||
Peptide sequence † | FEGGPFFNPDHDK | |||
| | | | | |: | ||||
Hypothetical oxido reductase (P77256) | ..AIGGGPAWNGDLDR.. |
Determined by MALDI-TOF mass spectrometry.
Determined by nanoESI-IT mass spectrometry. Notice that by this method amino acids I and L, and K and Q are indistinguishable. For simplicity, only one of each are indicated; in this table we have chosen the amino acid which gives sequence identity with the indicated proteins. Since peptides are generated by trypsin in conditions where almost complete digestion is expected, we have assumed that K must be present instead of Q at C-terminal position, and that Q is more probable than K in internal positions.
Only a partial sequence of this peptide could be determined; therefore, the observed mass does not agree with the depicted sequence.