Fig. 5.

Single anti-B7-1 or anti-B7-2 MoAb treatment can inhibit ANoA induction In Hg-induced autoimmunity. Groups of five mice received HgCl₂ and antibody injections as detailed in protocol 2 (Fig. 1). ANoA were detected by immunofluorescence on HEp-2 cells using isotype-specific FITC conjugates [14]. Results are expressed as serum titres (inverse of the highest serum dilution that yielded nucleolar fluorescence) ± s.d. None of the mice receiving either anti-B7-1 MoAb alone or both anti-B7-1 and anti-B7-2 MoAbs developed ANoA. Although some anti-B7-2-treated mice developed ANoA after week 3, their titres were significantly (P < 0·05) lower than those in the control groups. □, Anti-B7-1; ▵, anti-B7-2; ○, anti-B7-1 + anti-B7-2; ♦, isotype control; ▪, HgCl₂ alone.