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Gel mobility shift assay of the HNF1 binding sites. Gel mobility shift assays were carried out as described in Materials and Methods. The same amount of purified HNF1 (50 ng) and 32P-labeled oligonucleotide (3 × 10 5cpm) corresponding to the sequence of the 9-kb fragment in boxes 1 and 2 was used in the assay. For each oligonucleotide, assays were performed in triplicate.
