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Activation of the heterologous thymidine kinase promoter by human PAH HNF1 binding sites. The 0.5-kb BamHI–XbaI fragment containing human HNF1 binding sites were inserted upstream of thymidine kinase protomer of the luciferase vector pTK-Luc to construct pTK-Luc-HNF1. HNF1 response was examined by cotransfecting CHO cells with pTK-Luc-HNF1 in the presence or absence of pBJ5-HNF1. The data shown are means ± SD (n = 3).
