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Indirect immunofluorescence detection of CLIP-170 autoantibodies on HEp-2 cells. (a) IIF staining pattern of index human serum demonstrating reactivity in the cytoplasm as well as the nuclear matrix. Original magnification 100×. (b) Higher power (500x) of Hep-2 cells stained with the prototype sera demonstrating the dispersed and polymorphous cytoplasmic speckled staining pattern. In some areas the staining appears more punctate (arrows).
