Fig. 4.

Immunoblotting profile of anti CLIP-170 serum. (a) HeLa whole cell extracts were separated by 10% gel SDS-PAGE and transferred to nitrocellulose. Lane 1 shows the reactivity of the index human serum with a polypeptide of approximately Mr 170. Lane 2 shows the reactivity with antibodies from the index patient serum affinity purified on recombinant phage protein. Lane 3 shows the lack of reactivity of normal human serum. (b) Reactivity with the VLK2·1 recombinant protein produced in Escherichia coli was evident using the index serum (lane 1) and with affinity purified antibodies (lane 2), but normal human serum (lane 3) did not react with the recombinant protein. All sera were diluted 1/100, except affinity purified antibodies that were used undiluted. Molecular weight markers are shown on the left (a) and right (b).