Fig. 1.

Flow cytometric analysis of propidium iodide (PI)-stained HUVECs. After the HUVECs were stimulated with or without TNF-α (100 ng/ml) for 6 h, the cells were incubated in the presence and absence of IVIG (20 mg/ml) for 36 h. Representative histograms of the DNA content in HUVECs from a single donor are demonstrated. The percentage (%) of the cells with hypodiploid DNA was regarded as the proportion of apoptotic cells.