Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Mar;127(3):445–454. doi: 10.1046/j.1365-2249.2002.01769.x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2002 Blackwell Science Ltd

PMC Copyright notice

Fig. 3 — Time- and dose-dependent effects of IVIG on HUVEC apoptosis. (a) TNF-α (100 ng/ml)-stimulated or unstimulated HUVECs were incubated in the presence and absence of IVIG (20 mg/ml) for 72 h. The percentage of cells with hypodiploid was plotted in 0, 6, 12, 24, 36, 48 and 72 h after the addition of IVIG. •, Unstimulated; ○, unstimulated + IVIG; ■, TNF-α only; □, TNF-α + IVIG. *P < 0·05 versus unstimulated, unstimulated + IVIG and TNF-α only. (b) After the HUVECs were stimulated with TNF-α (0, 1, 10, 50, 100 and 500 ng/ml) for 6 h, the cells were incubated with IVIG (5, 10, 20, 30 and 40 mg/ml) for 24 h. The control indicates no treatment with IVIG. *P < 0·05 versus control in the same group. The data were expressed as the mean of samples ± SD from five experiments. □, Control; , IVIG: 5 mg/ml; , IVIG: 10 mg/ml; , IVIG: 20 mg/ml; ■, IVIG: 30 mg/ml; , IVIG: 40 mg/ml.

Inline graphic — Time- and dose-dependent effects of IVIG on HUVEC apoptosis. (a) TNF-α (100 ng/ml)-stimulated or unstimulated HUVECs were incubated in the presence and absence of IVIG (20 mg/ml) for 72 h. The percentage of cells with hypodiploid was plotted in 0, 6, 12, 24, 36, 48 and 72 h after the addition of IVIG. •, Unstimulated; ○, unstimulated + IVIG; ■, TNF-α only; □, TNF-α + IVIG. *P < 0·05 versus unstimulated, unstimulated + IVIG and TNF-α only. (b) After the HUVECs were stimulated with TNF-α (0, 1, 10, 50, 100 and 500 ng/ml) for 6 h, the cells were incubated with IVIG (5, 10, 20, 30 and 40 mg/ml) for 24 h. The control indicates no treatment with IVIG. *P < 0·05 versus control in the same group. The data were expressed as the mean of samples ± SD from five experiments. □, Control; , IVIG: 5 mg/ml; , IVIG: 10 mg/ml; , IVIG: 20 mg/ml; ■, IVIG: 30 mg/ml; , IVIG: 40 mg/ml.