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. 2002 Mar;127(3):445–454. doi: 10.1046/j.1365-2249.2002.01769.x

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© 2002 Blackwell Science Ltd

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Fig. 7 — Levels of intracellular ROS and effect of antioxidants on IVIG-induced HUVEC apoptosis. (a) After the HUVECs were stimulated with or without TNF-α (100 ng/ml) for 6 h, the cells were incubated in the presence and absence of IVIG (20 mg/ml) for 6 h. Thereafter, the HUVECs were exposed to DCF-DA and were observed under confocal laser microscopy. (b) After HUVECs were stimulated with or without TNF-α (100 ng/ml) for 6 h, the cells were treated with NAC (30 mm) and catalase (250 U/ml) for 1 h, followed by the incubation with IVIG (20 mg/ml) for 36 h. The control indicates no treatment with NAC or catalase. *P < 0·05 versus control. □, Control; , NAC; ■, catalase; , NAC + catalase.

Inline graphic — Levels of intracellular ROS and effect of antioxidants on IVIG-induced HUVEC apoptosis. (a) After the HUVECs were stimulated with or without TNF-α (100 ng/ml) for 6 h, the cells were incubated in the presence and absence of IVIG (20 mg/ml) for 6 h. Thereafter, the HUVECs were exposed to DCF-DA and were observed under confocal laser microscopy. (b) After HUVECs were stimulated with or without TNF-α (100 ng/ml) for 6 h, the cells were treated with NAC (30 mm) and catalase (250 U/ml) for 1 h, followed by the incubation with IVIG (20 mg/ml) for 36 h. The control indicates no treatment with NAC or catalase. *P < 0·05 versus control. □, Control; , NAC; ■, catalase; , NAC + catalase.