Fig. 8.

Cytofluorometric analysis of the mitochondrial transmembrane potential (δψ_m).After the HUVECs were stimulated with or without TNF-α (100 ng/ml) for 6 h, the cells were incubated in the presence (bold lines) and absence (shaded histograms) of IVIG (20 mg/ml) for 24 and 36 h. After the HUVECs were exposed to DiOC₆ and analysed by a flow cytometer. The proportion (%) of cells in M₁ indicates the loss of δψ_m, represented by a decrease in the fluorescence (FL1-H). As a positive control, CCCP (50 μm) was able to cause a complete loss of δψ_m at 0 h (dotted lines). All data are representative of five independent experiments giving similar results. , IVIG (−); —, IVIG (+); …, positive control.