Fig. 9.
Caspase activation and the effect of caspase inhibitors on the IVIG-induced HUVEC apoptosis.HUVECs were stimulated with or without TNF-α (100 ng/ml) for 6 h, the cells were treated with caspase inhibitors (10 μm) for 1 h, followed by incubation with IVIG (20 mg/ml) for 36 h. The percentage of cells with hypodiploid DNA was expressed as the mean of the samples ± SD from five experiments. C.I., non-specific caspase inhibitor; C.I.3, caspase-3 inhibitor; C.I.8., caspase-8 inhibitor; C.I.9, caspase-9 inhibitor. The control indicates no treatment with caspase inhibitors. *P < 0·05 versus control. □, Control; ■, C.I.;
, C.I.3;
, C.I.8;
, C.I.9;
, C.I.8 + C.I.9.
