Table 1.
Autoantibodies against cytochrome P450s among children on immunosuppressive drugs
| Adults | Children | ||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CyA or FX | No drug | CyA | CyA switched to FX | FX | |||||||||||||||||||||
| Number CYP pos. | Number CYP pos. | Number CYP pos. | Number CYP pos. | Number CYP pos. | |||||||||||||||||||||
| Pos. | 2E1 | 3A4 | 1A2 | 2C9 | Pos. | 2E1 | 3A4 | 1A2 | 2C9 | Pos. | 2E1 | 3A4 | 1A2 | 2C9 | Pos. | 2E1 | 3A4 | 1A2 | 2C9 | Pos. | 2E1 | 3A4 | 1A2 | 2C9 | |
| Kidney transplant | 7 | 1 | 1 | − | − | 0 | − | − | − | − | 21 | 1 | 5 | 1 | 2 | 29 | 1 | 2 | 1 | − | 25 | 1 | 1 | − | − |
| Nephrotic/kidney transplant | − | − | − | − | − | 0 | − | − | − | − | 20 | − | 1 | − | − | 50 | − | 1 | − | − | 0 | − | − | − | − |
| Nephrotic/ | − | − | − | − | − | 20* | − | − | 1 | − | 11 | − | − | − | 1 | 0 | − | − | − | − | 0 | − | − | − | − |
| Liver disease transplant | − | − | − | − | − | 0 | − | − | − | − | 0 | − | − | − | − | 0 | − | − | − | − | 0 | − | − | − | − |
| Liver disease before | − | − | − | − | − | 6 | − | 1 | − | − | 0 | − | − | − | − | 0 | − | − | − | − | 0 | − | − | − | − |
| Controls | − | − | − | − | − | 8 | − | 1 | 1 | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − |
| Total | 7 | 1 | 1 | 0 | 0 | 8.5 | 0 | 2 | 2 | 0 | 16 | 1 | 6 | 1 | 3 | 31 | 1 | 3 | 1 | 0 | 21 | 1 | 3 | 0 | 0 |
| †IB intensity | 28(25–30) | 32(5–70) | 105(26–245) | 95(15–240) | 478–140 | ||||||||||||||||||||
The positive ECL-immunoblot IgG reactions against human cytochrome P450s 2E1, 3A4, 1A2 or 2C9 with 1:600 diluted sera from adults (n = 30) and children undergoing drug treatments cyclosporin A (CyA) (n = 67), CyA and tacrolimus FK506 (FK) (n = 16) or FK alone (n = 14). Pos, Population that gave positive anti-CYP reactivities in immunoblots.
*
The group included three patients on prednisone.
†
The immunoblot (IB) reactivities were quantified by laser scanning of the autoradiographs and densitometric analysis using Molecular Dynamics Image Quant software version 3.2. The values shown are the mean and range (parentheses) of band intensities in a.u./pmol P450.