Fig. 1.

Schematic representation of the HAV genome and ssRNA constructs used for transfection. Restriction enzyme sites within the full-length cDNA construct pHAV38-2 [19,20] are shown. In vitro transcription following digestion with HaeII yields full-length RNA (full), whereas XhoI or EcoRI yields truncated RNAs (ΔX and ΔE, respectively) that do not contain the region essential for RNA-dependent RNA synthesis [19,20].