Fig. 3.

Northern blot analysis of MHC class I, LMP2 and TAP1 following transfection with HAV ssRNA constructs. Full-length or truncated HAV ssRNA was generated by in vitro transcription from template as shown in Fig. 1 and transfected into Hu-H7 cells. Total cellular RNA was prepared at the indicated time points, and Northern blotting performed as described in Materials and Methods. Mock transfection was performed using water instead of HAV ssRNA.