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Induction of MHC class I mRNA (a) and cell surface protein (b) by dsRNA in Hu-H7 cells. Hu-H7 cells were transfected with ssRNA (poly A) or dsRNA (poly IC-poly IC). Northern blot analysis (a) and FACS analysis (b) were performed 24 and 48 h after transfection, respectively. Mock transfection with RNase-free water served as a negative control and human recombinant IFNγ (100 U/ml) as a positive control. Numbers in parentheses in (b) are mean fluorescence intensity.
