Fig. 5.

The PKR inhibitor 2AP, as well as MMI and pMMI, abolishes dsRNA-initiated MHC induction in Hu-H7 cells. After transfection of Hu-H7 cells with dsRNA (poly IC-poly IC), 2AP at final concentrations of 0·01, 0·1, 1·0 or 10 mm (lanes 3–6), 0·01, 0·1, 1·0 or 10 mm of MMI (lanes 7–10) or pMMI at concentrations of 0·01, 0·1 or 0·5 mm, were added to the culture medium. Total cellular RNA was prepared 48 h after transfection. The effect of 2AP is not due to altered transfection efficiency (see text).