Table 2.

Effect of anticytokine MoAbs on Streptococcus suis-inducedcytokine release by IFN-γ pre-activated THP-cells ^†

	% of inhibition ‡
S. suis-induced	Anti-TNF	Anti-IL-1	Anti-TNF + Anti-IL-1	Anti-MCP
IL-1	33 ± 6*	NT	NT	0 ± 0
IL-6	17 ± 3	1 ± 1	31 ± 7*	0 ± 0
IL-8	31 ± 5*	5 ± 4	28 ± 3*	6 ± 4
MCP-1	35 ± 3*	35 ± 9*	65 ± 4**	NT

^†IFN-γ pre-activated THP-1 cells were cultured in the presence of antihuman TNF-α (40 μg/ml), IL-1β (15 μg/ml) or MCP-1 (5 μg/ml) MoAbs; or the combination of both TNF-α + IL-1β MoAbs. Neutralizing MoAbs were added at the beginning of culture together with the bacterial stimulus (heat-killed 31533 strain at 10⁹ cfu/ml) for 24 h.

^‡Data are mean ± s.e.m. NT: not tested.

Significant inhibition (by the Student’s unpaired t-test) respect to values for bacterial stimulus without treatment (100% of production).

^*P < 0·01;

^**P < 0·001.