Fig. 6.

Chemotactic migration of activated HMC in response to MCP-1. Growth-arrested cells were either untreated or activated with IFNγ (1000 U/ml) for 24 h, detached, recovered as described in Materials and Methods, and loaded into the upper compartment of a microchemotaxis chamber (20 000 cells/well). The chemotactic factors, MCP-1 and platelet-derived growth factor (PDGF), were loaded at the indicated concentrations into the lower compartment, and the two compartments were separated using a 14 μm pore size polycarbonate membrane. After a 3 h incubation at 37°C, migrated cells were fixed and stained. For each data point, three high-power fields were counted. (□) Medium; (▪) IFNγ. The results represent the average numbers plus s.e.m. of four experiments.