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. 2002 Jul;129(1):86–91. doi: 10.1046/j.1365-2249.2002.01895.x

Fig. 5.

Fig. 5

EMSA assay of NF-κB and NF-IL-6 activation after exposure to pressure in IEC-18 cells. (a) NF-κB DNA binding activity as determined by elecrophoretic mobility shift assay (EMSA) using a FITC-labelled consensus NF-κB oligonucleotide as a probe. Left: control IEC-18 cells extracts (lane 1), and 1 and 2h after exposure to a pressure of 80 mmHg (lanes 2 and 3). Right: the specificity of NF-κB binding was confirmed with the competition by cold oligonucleotides. (b) EMSA assay of NF-IL-6 DNA binding activity after exposure to pressure in IEC-18 cells. Left: control IEC-18 cells extracts (lane 1), and 1 and 2h after the exposure of pressure at 80 mmHg (lanes 2 and 3). Right: the specificity of NF-IL-6 binding was confirmed by cold oligonucleotides.