Fig. 4.
Treatment with anti-CD154 MoAb significantly inhibits IL-12 mRNA. B6 mice received 2 × 107 irradiated BALB/c splenocytes and MoAb treatment as described in the Methods. Total RNA from splenocytes at the indicated time after transfer was isolated and expression of IL-12 p40 mRNA was assessed by RT-PCR as described in the Methods. (a) Kinetic change in IL-12 p40 mRNA expression after transfer. The predicted product sizes for IL-12 p40 and β2-microglublin were 384 and 222 bp, respectively. (b) Comparison of IL-12 p40 mRNA at 6 h after transfer. The amount of mRNA for IL-12 p40 and β2-microglobulin in individual samples at 6 h after transfer was measured by densitograph and the unit values were normalized to the amount of mRNA for β2-microglobulin. The bars represent the mean ± s.d. from each group of seven to eight mice. *Statistically different (P < 0·05).