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Aberrant FasL protein expression on peripheral blood lymphocytes from SLE patients. Freshly isolated PBMC from four SLE patients were used either immediately (0) or stimulated by 3 h culture with PHA (3). As a control, normal PBMC were stimulated with PHA for up to 15 h. An equal amount of proteins from the cell lysates was analysed by immunoblotting using affinity-purified antihuman FasL fragment 5 antibody. Results shown are representative of six independent experiments.
