Fig. 1.

Comparative study of cytokeratin 8/18, vimentin and SV40 large T antigen expression in HPMC culture. Monolayer T-HPMC (passage 8) (a, c, e) and P-HPMC (passage 3) (b, d, f) were incubated with anticytokeratin 8/18, vimentin and SV40 large T antigen antibody, respectively, detected by Vectastain® UNIVERSAL Elite ABC KIT. Co-expression of cytokeratin 8/18 (a, b) and vimentin (c, d) was observed on both T-HPMC and P-HPMC culture. Nearly all T-HPMC exhibit an intense nuclear expression of SV40 large T antigen (e), whereas P-HPMC show no detectable expression of this antigen (f).