Fig. 5.

TGF-β production was increased in metallothionein-injected mice. Lymph node cells obtained on day 34 post mortem were cultured in RPMI medium supplemented with 1·25% FBS in the absence (□) or presence (▪) of 250 μg/ml bovine CII for 3 days. The culture supernatants were collected and analysed for TGF-β levels using cytokine sandwich ELISA. The result of a representative experiment out of three performed is shown.