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. 2002 Aug;129(2):318–325. doi: 10.1046/j.1365-2249.2002.01891.x

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© 2002 Blackwell Science Ltd

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Fig. 3 — Fine specificity of human IgG antibodies reactive with EB200. (a) Sera from 83 Liberian adults were analysed at a dilution of 1 : 1000 for antibody reactivity with EB200 and a panel of peptides together spanning the EB200 sequence in ELISA. The reactivity was defined as percent reactivity of a human immune serum reactive with peptide 6 × 4 and was graded as below cut off, >cut off 15%, >15%–25% and >25%. Cut-off for each ELISA antigen was defined as the mean reactivity of Swedish control sera +3 s.d. (b) Antibodies affinity purified on peptide P-2, -6, -12 or -17 were tested for reactivity with EB200 as well as with P-1 to -17 in ELISA. Reactivity with EB200 and peptides displayed by serum from donor BH147 diluted 1 : 1000 and affinity purified IgG from the same donor diluted 1 : 300. (c) Peptide specificity of antibodies affinity purified on P-17 from donor BD67 and W150, respectively. (b and c) The y axes present the ELISA absorbance values at 405 nm. Reactivities of >50% of the reactivity with the peptide used for purification are indicated with black bars. Reactivities of less than 50% are depicted with white bars. Asterisks indicate the peptide used for purification. □, ≤Cut-off; , <cut-off 15%; , >15–25%; ▪, >25%.

Inline graphic — Fine specificity of human IgG antibodies reactive with EB200. (a) Sera from 83 Liberian adults were analysed at a dilution of 1 : 1000 for antibody reactivity with EB200 and a panel of peptides together spanning the EB200 sequence in ELISA. The reactivity was defined as percent reactivity of a human immune serum reactive with peptide 6 × 4 and was graded as below cut off, >cut off 15%, >15%–25% and >25%. Cut-off for each ELISA antigen was defined as the mean reactivity of Swedish control sera +3 s.d. (b) Antibodies affinity purified on peptide P-2, -6, -12 or -17 were tested for reactivity with EB200 as well as with P-1 to -17 in ELISA. Reactivity with EB200 and peptides displayed by serum from donor BH147 diluted 1 : 1000 and affinity purified IgG from the same donor diluted 1 : 300. (c) Peptide specificity of antibodies affinity purified on P-17 from donor BD67 and W150, respectively. (b and c) The y axes present the ELISA absorbance values at 405 nm. Reactivities of >50% of the reactivity with the peptide used for purification are indicated with black bars. Reactivities of less than 50% are depicted with white bars. Asterisks indicate the peptide used for purification. □, ≤Cut-off; , <cut-off 15%; , >15–25%; ▪, >25%.