Table 1.
Variation in the number of whole lymphocytes and NK1·1 + TCRint cells (NKT cells) by the administration of denatured liver tissue
| No. of whole lymphocytes | No. of NKT cells | |||||
|---|---|---|---|---|---|---|
| Day after the injection of denatured liver tissue | Liver (×106) | Spleen (×107) | Thymus (×107) | Liver (×105) | Spleen (×106) | Thymus (×105) |
| – | 4·6 ± 0·6 | 7·6 ± 0·9 | 15·8 ± 1·2 | 8·5 ± 1·6 | 2·0 ± 1·8 | 8·4 ± 1·0 |
| day 1 | 2·1 ± 0·8* | 4·1 ± 0·5* | 7·9 ± 1·0* | 6·0 ± 1·5 | 1·2 ± 0·9* | 6·8 ± 0·6 |
| day 7 | 3·2 ± 0·6* | 5·5 ± 0·6* | 8·0 ± 1·0* | 7·4 ± 1·4 | 1·9 ± 0·9 | 3·9 ± 1·2* |
| day14 | 5·8 ± 0·8 | 8·0 ± 1·0 | 11·5 ± 1·1 | 13·0 ± 1·2* | 2·2 ± 0·8 | 4·6 ± 0·9* |
| day21 | 5·0 ± 0·7 | 9·8 ± 1·0 | 9·8 ± 0·9 | 10·1 ± 1·2* | 2·3 ± 0·8 | 9·9 ± 0·8 |
Lymphocytes were obtained from the liver on days1, 7, 14 and 21 after the injection. Two-colour stainings for NK 1·1 and CD3 were conducted to identify NKT cells (NK1·1+ CD3int cells). The absolute number (per one mouse) of NKT cells was estimated by calculation using the number of total lympocytes and the proportion of NKT cells.
The mean and one SD are represented (n = 4 at each point of time).
*
P < 0·05.