Table 3.
Cytokines (pg/ml) | ||||||||
---|---|---|---|---|---|---|---|---|
IL-2 | IL-4 | IL-5 | IFN-γ | |||||
Donor | – | Peptide | – | Peptide | – | Peptide | – | Peptide |
1. DR1/DR1; DQ6/DQ3 | <250 | 1000 | <250 | <250 | <250 | <250 | <250 | 2300 |
2. DR1/DR3; DQ5/DQ2 | <250 | <250 | <250 | <250 | <250 | <250 | <250 | 2500 |
3. DR3/DR4; DQ2/DQ3 | <250 | 2400 | <250 | <250 | <250 | <250 | <250 | <250 |
4. DR3/DR11; DQ2/DQ3 | <250 | <250 | <250 | <250 | <250 | <250 | <250 | 7500 |
5. DR3/DR11; DQ2/DQ3 | <250 | <250 | <250 | <250 | <250 | <250 | <250 | 2500 |
6. DR4/DR7; DQ2/DQ5 | <250 | <250 | <250 | <250 | <250 | <250 | <250 | 2000 |
7. DR4/DR13; DQ3/DQ6 | <250 | 2400 | <250 | <250 | <250 | <250 | <250 | 1800 |
8. DR4/DR13; DQ3/DQ3 | <250 | 7500 | <250 | <250 | <250 | <250 | <250 | 10000 |
9. DR7/DR9; DQ2/DQ3 | <250 | 5000 | <250 | <250 | <250 | <250 | <250 | 20000 |
10. DR13/DR8; DQ6/DQ4 | <250 | 8500 | <250 | <250 | <250 | <250 | <250 | 2500 |
11. DR13/DR11; DQ3/DQ3 | <250 | <250 | <250 | <250 | <250 | <250 | <250 | 2600 |
12. DR15/DR3; DQ6/DQ2 | <250 | <250 | <250 | <250 | <250 | <250 | <250 | 3100 |
13. DR15/DR4; DQ6/DQ3 | <250 | 7000 | <250 | <250 | <250 | <250 | <250 | 10000 |
14. DR15/DR13; DQ6/DQ6 | <250 | <250 | <250 | <250 | <250 | <250 | <250 | 8000 |
IL-2, IL-4, IL-5 and IFN-γ productions were evaluated in 48 h-culture supernatant of CD4+ T cells immunized or not ex vivo with 50 μg/ml of Nef 56–68 peptide in the presence of autologous DCs as APCs (n = 14 donors).