Fig. 5.

IFN-γ and IL-4 mRNA expression in BALF T cells from DM/PM patients with corticosteroid-resistant and corticosteroid- sensitive IP.IFN-γ and IL-4 mRNA expression was detected by PCR amplification of cDNA from BALF T cells in DM/PM patients with corticosteroid-resistant IP (n = 6) and corticosteroid-sensitive IP (n = 16) and controls without IP (n = 12). TCR Cβ mRNA expression was used as an internal control of PCR amplification of cDNA. The PCR products were run on 1·5% agarose gel, and visualized using ethidium bromide. The data shown are representative of corticosteroid-resistant IP (lanes 1–4), corticosteroid-sensitive IP (lanes 5–8), and controls without IP (lanes 9 and 10). P, activated peripheral blood T cells as a positive control.