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IgG antibodies to CCR5 in sera from normal controls (n = 10), allo-immunized women (n = 6) and HLA typing sera (n = 7). Serum antibodies were assayed by ELISA. Doubling dilutions of test samples were applied to plates coated with a predetermined optimal concentration of CCR5 antigen preparation (1 μg/ml) and the bound antibodies to CCR5 were detected by secondary rabbit antibody to human IgG, followed by affinity-purified goat antirabbit IgG-alkaline phosphatase conjugate.
