Table 2.
Functional assays of human serum IgG antibodies to CCR5 adsorbed with CCR5 transfected or untransfected HEK-293 cells
| % Inhibition of HIV replication | % Inhibition of chemotaxis | |||||
|---|---|---|---|---|---|---|
| Subject no. | Untreated | 293 | CCR5-293 | Untreated | 293 | CCR5-293 |
| 3637 | 75.9 | 65.7 | 23.1 | 72 | 91 | 52.5 |
| 3590 | 46.4 | 56.6 | 33.6 | 79.3 | 61.4 | 27.8 |
| 3690 | 66.3 | 77.4 | 13.8 | 70.2 | 51.1 | 50.3 |
| 3703 | 64 | 48.6 | 28.0 | ND | ND | ND |
| 3584 | 90.1 | 67.8 | 29.3 | 86.0 | 87.8 | 48.1 |
| 3595 | 89.3 | 69.9 | 56.7 | 55 | 78.6 | 16.9 |
| Mean (+sem) | 72.0 (6.2) | 64.3 (3.8) | 30.8 (5.4) | 72.5(4.6) | 73.9 (6.9) | 39.1 (6.3) |
| 1* | 8.3 | 12.9 | 8.1 | 3 | 6.4 | 36.2 (24.5) |
| 2* | 11.4 | 1.8 | 6.5 | 25.9 | 22.5 | 29.8 |
| 3* | 12.6 | 0 | 16.1 | 32.5 | 31.9 | 33.4 |
| Mean (+sem) | 10.8 (1.3) | 4.9 (4.0) | 10.2 (2.9) | 31.6 (3.1) | 30.2 (4.0) | 29.2 (2.6) |
*
Control sera from non-parous women showed no inhibiting antibodies.