Fig. 1.

DNA and protein sequences of 1E8-4b Fab in the pHFA² cloning vector. Both the heavy (V_HC_H1) and light (V_LC_L) chains of the 1E8-4b Fab are preceeded by N-terminal pelB signal sequences (italicised). Non-coding regions located 5′ to the pelB signal sequences are shown in lower case. C-terminal peptide tag sequences (tubulin (Glu Glu Phe) and Flag) are shown in bold. The framework, CDR and constant regions are outlined above the sequence, according to Kabat et al. [20]. Important restriction sites are underlined.