Fig. 3.

ELISA depicting the reactivity of the parent 1E8 antibody (a) and the recombinant 1E8-4b Fab fragment (b) to Aβ[1–40], Aβ [1–42], Aβ[1–43], APP695 and APP770 peptides. The antibody is titrated from 10 μg/ml with serial doubling dilutions, while the peptide is at a constant concentration of 50 ng/well. 1E8 was probed with antimouse-HRP while 1E8-4b was probed with M2 anti-Flag antibody and then with antimouse-HRP. Detection of antibody reactivity to the peptides was via an ‘o’-phenylenediamine development mixture. The isotype control used was M2 anti-Flag antibody which is a mouse IgG₁.