Fig. 6.

Immunohistochemistry of AD brain sections. Brain sections were taken from the same AD brain patient for accurate comparison. The 1E8-4b recombinant Fab fragment, the 1E8 antibody and the isotype control M2 anti-Flag antibody were applied to the sections at a concentration of 20 μg/ml at x20 (a) and x60 (b) magnification. At 10 μg/ml and 20 μg/ml the 1E8-4b and 1E8 antibodies showed reactivity to amyloid associated with plaques, while the isotype control showed no reactivity. Sections 1 and 4: 1E8; sections 2 and 5: 1E8-4b; sections 3 and 6: isotype control M2 anti-Flag monoclonal antibody.