Table 2.
Interaction of monoclonal and polyclonal antibodies with solid-phase GAD
| Dilution | O.D.405 | |
|---|---|---|
| (a) Binding of mouse MoAbs to rbGAD by direct ELISA | ||
| MoAb | ||
| N-terma | 1 : 500 | 0·122 |
| C-terma | 1 : 1000 | 0·187 |
| GAD-6b | 1 : 1000 | 0·177 |
| (b) Binding of rabbit polyclonal antibodies (C-pc antibody) to rbGAD by direct ELISA | ||
| Preparation | ||
| Ig enriched fractionc | 1 : 800 | 0·514 |
| Affinity purified fractiond | 1 : 500 | 0·253 |
| Affinity depleted fractione | 1 : 500 | 0·090 |
a
Partially purified immunoglobulin preparation from hybridoma culture supernatant.
b
Purified immunoglobulin: stock concentration 1 mg/ml.
c
Partially purified immunoglobulin precipitated from serum with caprylic acid and ammonium sulphate.
d
Eluted after binding to rbGAD coupled to Sepharose 4B.
e
Effluent not bound by rbGAD coupled to Sepharose 4B.