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. 2002 Oct;130(1):59–66. doi: 10.1046/j.1365-2249.2002.01921.x

Fig. 5.

Fig. 5

Transepithelial migration of neutrophils across T84 monolayers in response to apical stimulation by B. fragilis enterotoxin. T84 monolayers in transwell chamber were apically pretreated with NF-κB inhibitor MG-132 (50 μm) for 1 h, and then stimulated with B. fragilis enterotoxin (BFT, 100 ng/ml) for 6 h. The stimulated monolayers were transferred, apical side down, into a 24-well tissue culture tray. Isolated human neutrophils (106) were added to the basolateral side (upper reservoir) of each monolayer and incubated 2 h at 37°C. The number of neutrophils that had transmigrated was quantified by the myeloperoxidase assay. The potent chemotactic peptide fMLP (100 nm) was used as a positive control for these experiments. The negative control consisted of basolaterally positioned neutrophils in the absence of chemoattractive gradients. Data represent the mean ± SEM (n = 7). An asterisk indicates value significantly different from BFT-stimulated T84 monolayers without pretreated MG-132 (P < 0·05).