Fig. 6.

Nasal administration of CTB-insulin abolished the proliferative response to insulin immunization and not OVA immunization; 4-week-old NOD female were untreated (control) or nasally treated with 1 µg of CTB-insulin (nasal treatment). Ten days later, the mice were immunized with 50 µg of porcine insulin (a) or 50 µg of OVA (b). The basal proliferative response of splenic T cells (in white) was compared with the response to 20 µg/ml insulin (a) or OVA (b) (in black) 8 days after the immunization. **P < 0·0001.