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CD154 and the transmembrane form of TNF-α (mTNF-α) on activated MRL/lpr T cell lines. CD154-intact autoreactive Th1 (5-1), and Th2 (4-1) lines and CD154-deficient Th1 (P5) were stimulated with plate-bound anti-CD3ɛ MoAb (5 µg/ml) for the indicated time. CD154 was not expressed on P5 during 0–48 h stimulation with anti-CD3ɛ MoAb. Bold lines show staining with anti-CD154 or TNF-α MoAb, and regular lines show isotype control.
