Fig. 1.
Effect of in vivo anti-CTLA-4 MoAb administration on cell proliferation in orally tolerized mice. Mice were treated i.p. with either (a) rat IgG (75 µg/mouse) or (b) anti-CTLA-4 MoAb (75 µg/mouse) on days 0, + 1 and + 2 and fed OVA (1 mg/mouse) on days 0, + 1 and + 2. Ten days after feeding, mice were injected with 10 µg of OVA/IFA in the footpad and tail base and sacrificed 11 days later. Popliteal lymph node (PLN) cells were incubated in vitro with 1000 and 100 µg of OVA and proliferation was assessed using a BrdU cell proliferation colourimetric kit (see Materials and Methods). The error bars represent standard deviations calculated from quadruplicate samples. (a) ▪, Rat IgG/H2O; 
, rat IgG/OVA. (b) ▪, Anti-CTLA-4/H2O; , anti-CTLA-4/OVA.