Fig. 3.
Effect of in vivo anti-CTLA-4 MoAb and IL-12 administration on cell proliferation in orally tolerized mice. Mice were treated i.p. with either rat IgG (75 µg/mouse) or anti-CTLA-4 MoAb (75 µg/mouse) (a) or with IL-12 (1 µg/mouse) or anti-CTLA-4/IL-12 (b) on days 0, + 1 and + 2. Mice were fed OVA (1 mg/mouse) on days 0, + 1 and + 2. Ten days after feeding, mice were injected with 10 µg of OVA/IFA in the footpad and tail base and sacrificed 11 days later. PLN cells were removed and incubated in vitro with 1000 µg/ml or 100 µg/ml of OVA and proliferation was assessed using a BrdU cell proliferation colourimetric kit (see Materials and Methods section). The error bars represent stadard deviations calculated from quadruplicate samples. In (a): ▪, Rat IgG/H2O; □, rat IgG/OVA; 
, anti-CTLA-4/H2O;
, anti-CTLA-4/OVA. In (b): ▪, IL-12/H2O; □, IL-12/OVA; , IL-12/anti-CTLA-4/H2O; , IL-12/anti-CTLA-4/OVA.