Table 2.

Effect on cytokine release of increasing the coating concentration of PPS coated onto latex beads. Blood sample from a healthy, blood group O subject was incubated at 37°C with PPS (10 μg/ml), PPS-coated beads at concentrations of 16·6 μg, 166 μg and 332 μg/10⁸ beads and uncoated beads (1 × 10⁸ beads/ml blood), sampled at 0, 3, 6 and 8 h and analysed cytokine release. The values shown represent the mean of duplicate determinations

	0 h	3 h	6 h	8 h
IL-6 pg/ml
Control (blood)	12	13	31	43
PPS.S14	12	62	1371	2829
Beads only	15	20	38	90
16·6 μg PPS/108 beads	9	14	36	58
166 μg PPS/108 beads	9	18	42	62
332 μg PPS/108 beads	10	23	60	84
TNFα pg/ml
Control (blood)	22	25	24	24
PPS.S14	14	472	1085	1118
Beads only	34	23	30	35
16·6 μg PPS/108 beads	11	23	41	28
166 μg PPS/108 beads	15	24	37	23
332 μg PPS/108 beads	12	32	32	30