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. 2002 Dec;130(3):559–561. doi: 10.1046/j.1365-2249.2002.02041.x

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© 2002 Blackwell Publishing Ltd

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Fig. 3 — Time-course effect of 4AP on TNF-α production by human MACs. 4AP, at a final concentration of 5 mm, was added to the culture medium at the different time points (15 min before, same time or 15 min, 30 min, 60 min or 120 min after LPS or PMA activation). MACs were then cultured for the remaining time period to 24 h. The media were harvested for TNF-α measurement by ELISA. These data illustrate the results from one of three individual experiments and represent the mean value of triplicate samples ± 1 standard deviation. The bar key, LPS × 10 shows that the real concentrations of TNF-α by LPS-activated MACs are 10-fold of the bar indicated in the graph. ▪, LPS × 10 (1 µg/ml); , PMA (40 nm).

Inline graphic — Time-course effect of 4AP on TNF-α production by human MACs. 4AP, at a final concentration of 5 mm, was added to the culture medium at the different time points (15 min before, same time or 15 min, 30 min, 60 min or 120 min after LPS or PMA activation). MACs were then cultured for the remaining time period to 24 h. The media were harvested for TNF-α measurement by ELISA. These data illustrate the results from one of three individual experiments and represent the mean value of triplicate samples ± 1 standard deviation. The bar key, LPS × 10 shows that the real concentrations of TNF-α by LPS-activated MACs are 10-fold of the bar indicated in the graph. ▪, LPS × 10 (1 µg/ml); , PMA (40 nm).